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Related Experiment Videos

Developmental changes in alpha-tocopherol, especially during infantile period

H Tamai1, H S Kim, H Arai

  • 1Department of Pediatrics, Osaka Medical College, Japan.

Zhonghua Minguo Xiao Er Ke Yi Xue Hui Za Zhi [Journal]. Zhonghua Minguo Xiao Er Ke Yi Xue Hui
|February 25, 1998
PubMed
Summary

Alpha-tocopherol transfer protein (alpha-TTP) expression in neonatal rat liver increases after birth, correlating with rising plasma alpha-tocopherol levels. This suggests alpha-TTP

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Area of Science:

  • Biochemistry
  • Developmental Biology
  • Nutritional Science

Background:

  • Alpha-tocopherol transfer protein (alpha-TTP) plays a crucial role in vitamin E metabolism.
  • Understanding the developmental regulation of alpha-TTP is essential for comprehending vitamin E homeostasis.

Purpose of the Study:

  • To investigate the developmental changes in alpha-TTP expression in neonatal rats.
  • To explore the relationship between alpha-TTP expression and plasma tocopherol levels.
  • To examine factors influencing alpha-TTP expression in cultured hepatocytes.

Main Methods:

  • Developmental expression analysis of alpha-TTP in rat liver post-birth.
  • Measurement of plasma alpha-tocopherol and gamma-tocopherol levels.
  • Primary culture of rat hepatocytes to study alpha-TTP expression.

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  • Western and Northern blot analysis in vitamin E-deficient and -enriched rats.

    • Main Results:

    • Neonatal rat liver alpha-TTP expression increased post-birth, reaching adult levels by 4 weeks.
    • Plasma alpha-tocopherol to gamma-tocopherol ratio increased linearly during suckling, correlating with alpha-TTP development.
    • Hepatocyte culture showed low alpha-TTP expression, modulated by epidermal growth factor and dexamethasone.
    • Vitamin E status influenced alpha-TTP mRNA levels, indicating regulation of its synthesis.

    Conclusions:

    • Hepatic alpha-TTP expression is developmentally regulated after birth.
    • The developmental increase in alpha-TTP expression likely contributes to the rise in plasma alpha-tocopherol levels.
    • Hepatic differentiation state and vitamin E availability may influence alpha-TTP expression.