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The use of reference materials in quantitative analyses based on FT-Raman spectroscopy
A T Paepe1, J M Dyke, P J Hendra
1Department of Chemistry, University of Southampton, Hants, UK.
Summary
External reference samples effectively standardize Raman scattering spectra intensities for quantitative analysis. This method provides reliable results with low variance, making it suitable for most applications.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Physical Chemistry
Background:
- Quantitative Raman spectroscopy relies on consistent spectral intensity measurements.
- Standardization is crucial for accurate data interpretation and inter-laboratory comparisons.
- External reference samples offer a potential method for achieving spectral intensity standardization.
Purpose of the Study:
- To assess the reliability of using external reference samples for standardizing Raman scattering spectra intensities.
- To evaluate the effectiveness of external standards in quantitative Raman analysis.
- To determine the precision of measurements using external standardization.
Main Methods:
- Comparison of intensity ratios within a two-component liquid mixture.
- Comparison of intensity ratios between the mixture and an external reference standard.
- Raman spectra recorded using modest laser powers over 2-minute acquisition times.
Main Results:
- External reference samples are highly adequate for standardizing Raman spectra in quantitative analysis.
- Coefficients of variance for repeated measurements on the same sample are approximately 1% when using single band heights.
- Using the entire spectrum for analysis yields significantly lower coefficients of variance.
Conclusions:
- External reference samples provide a reliable and effective means for standardizing Raman scattering spectra.
- The proposed method ensures high precision for quantitative Raman analysis.
- Whole spectrum analysis offers superior precision compared to single band analysis when using external standards.