Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

tRNA-dependent amino acid transformations

A W Curnow1, K W Hong, R Yuan

  • 1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA.

Nucleic Acids Symposium Series
|January 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measurement of the Positive Muon Anomalous Magnetic Moment to 127 ppb.

Physical review letters·2025
Same author

Measurement of the Positive Muon Anomalous Magnetic Moment to 0.20 ppm.

Physical review letters·2023
Same author

Measurement of the Positive Muon Anomalous Magnetic Moment to 0.46 ppm.

Physical review letters·2021
Same author

Identification of OAT1/OAT3 as Contributors to Cisplatin Toxicity.

Clinical and translational science·2017
Same author

Effects of Polyamines on TNFalpha- or Tamoxifen-induced Apoptosis in Human Breast Cancer Cells.

Cancer research and treatment·2015
Same author

Overcoming Challenges in Engineering the Genetic Code.

Journal of molecular biology·2015
Same journal

Nucleotide specificity of DNA cleavage by esperamycin/calichemicin antitumor antibiotics.

Nucleic acids symposium series·2018
Same journal

A model reaction for the repair of nucleic acids. The repair of thymidine bromohydrins.

Nucleic acids symposium series·2018
Same journal

Effects of base damages on DNA replication.

Nucleic acids symposium series·2018
Same journal

Effect of serine residue on the effectiveness of cationic polypeptide-based gene delivery.

Nucleic acids symposium series·2003
Same journal

Smart probe: a novel fluorescence quenching-based oligonucleotide probe carrying a fluorophore and an intercalator.

Nucleic acids symposium series·2003
Same journal

The engineering, structure, and DNA binding properties of a novel His4-type zinc finger peptide.

Nucleic acids symposium series·2003
See all related articles

This study purified the Glu-tRNA(Gln) amidotransferase enzyme from Bacillus subtilis. This enzyme is involved in indirect aminoacyl-tRNA synthesis and may be a therapeutic target.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Aminoacyl-tRNAs are essential for protein synthesis.
  • Synthesis occurs directly via aminoacyl-tRNA synthetases or indirectly through tRNA modification.
  • Enzymes in indirect synthesis pathways are potential therapeutic targets.

Purpose of the Study:

  • To purify and biochemically characterize the Glu-tRNA(Gln) amidotransferase from Bacillus subtilis.
  • To investigate enzymes involved in the indirect synthesis of aminoacyl-tRNAs.

Main Methods:

  • Purification of Glu-tRNA(Gln) amidotransferase from Bacillus subtilis to homogeneity.
  • Initial biochemical characterization of the purified enzyme.

Main Results:

Related Experiment Videos

  • Successful homogeneous purification of Bacillus subtilis Glu-tRNA(Gln) amidotransferase.
  • Generation of initial biochemical data for the purified enzyme.

Conclusions:

  • The purified Glu-tRNA(Gln) amidotransferase provides a basis for further biochemical studies.
  • This enzyme represents a potential target for novel therapeutic compound development.