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Antisense micF RNA and 5'-UTR of the target ompF RNA: phylogenetic conservation of primary and secondary structures

N Delihas1

  • 1Department of Molecular Genetics and Microbiology, School of Medicine, SUNY, Stony Brook, NY 11794-5222, USA.

Nucleic Acids Symposium Series
|January 1, 1997
PubMed

Insights

Antisense micF RNA regulates outer membrane protein F (OmpF) expression in E. coli by blocking translation and destabilizing OmpF mRNA. Conserved sequences facilitate binding, while conserved structures may involve protein interactions.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Outer membrane protein F (OmpF) is crucial for E. coli and related bacteria.
  • OmpF expression is regulated post-transcriptionally by antisense micF RNA.

Purpose of the Study:

  • To investigate the molecular mechanisms of micF RNA-mediated regulation of OmpF expression.
  • To analyze the sequence and structural conservation of micF RNA and OmpF mRNA.

Main Methods:

  • Phylogenetic analyses of micF RNA and OmpF mRNA sequences.
  • Analysis of RNA/RNA interaction regions and secondary structures.

Main Results:

  • MicF RNA binds to the 5' UTR of OmpF mRNA, inhibiting translation and promoting mRNA destabilization.
  • High nucleotide sequence conservation was observed in the RNA/RNA interaction regions.
  • Low sequence conservation but high secondary structure conservation was found in non-interacting regions.

Conclusions:

  • Conserved sequences in micF RNA and OmpF mRNA are essential for RNA/RNA binding and translational blockage.
  • Conserved secondary structures in non-interacting regions may mediate protein interactions involved in mRNA destabilization.

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