Identification and developmental expression of a Bombyx mori alpha-tubulin gene

A cDNA clone isolated from the wing discs at the metamorphosis of Bombyx mori during the period of morphogenesis has been characterized. The amino acid sequence predicted for the putative protein is highly homologous to the Drosophila alpha1-tubulin. This is the first alpha-tubulin gene isolated in Bombyx mori and other isotype sequences are present in the Bombyx genome. The transcript is detected in the wing discs at every postembryonic stage examined, and is also expressed in other tissues, but at different levels. Although the mRNA level is maximum when the 20-hydroxyecdysone titre is high, its accumulation is independent of the hormone level both in vivo and in vitro. Significance of the accumulation of the mRNA of an ubiquitous alpha-tubulin in developing wing discs is discussed by comparison with our knowledge of the alpha-tubulin family in Drosophila and in other organisms.