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[RNA typing of granulocyte NA antigens by means of PCR-RFLP analysis]
1Institut für Klinische Immunologie und Transfusionsmedizin, Justus-Liebig-Universität Giessen, Deutschland.
Beitrage Zur Infusionstherapie Und Transfusionsmedizin = Contributions to Infusion Therapy and Transfusion Medicine
|January 1, 1994
Abstract:
An RNA-based method was developed for genotyping of the granulocyte-specific alloantigens NA1 and NA2, mRNA was isolated from neutrophils and reversely transcribed into cDNA. To avoid coamplification of Fc-gamma receptor III-2 in the polymerase chain reaction (PCR), a Fc-gamma receptor III-1 sequence-specific primer was employed. Restriction analysis using Taq I led to NA-specific restriction fragment length polymorphism (RFLP) of PCR-amplified cDNA. The results of the PCR-RFLP typing were in good concordance with serological phenotyping by granulocyte immunofluorescence test and the antigen capture assay MAIGA.