Related Experiment Videos
[Two modifications of MAIPA assays for the demonstration of platelet antibodies]
V Kiefel1, S Scheld, E Freitag
1Institut für Klinische Immunologie und Transfusionsmedizin, Justus-Liebig-Universität Giessen, Deutschland.
The monoclonal antibody immobilization of platelet antigens assay (MAIPA), published in 1987, is now used by many laboratories for detection of platelet antibodies. False-positive results were observed with sera containing anti-mouse IgG and in cases where MAIPA was employed to characterize platelet-specific antibodies of the IgM class. Modifications of MAIPA which completely eliminate these artifacts are described.
The monoclonal antibody immobilization of platelet antigens assay (MAIPA), published in 1987, is now used by many laboratories for detection of platelet antibodies. False-positive results were observed with sera containing anti-mouse IgG and in cases where MAIPA was employed to characterize platelet-specific antibodies of the IgM class. Modifications of MAIPA which completely eliminate these artifacts are described.