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[In-line-filtration of erythrocyte concentrates using the Leukotrap-RC system]
E Richter1, G Matthes, U Tofoté
1Institut für Transfusionsmedizin, Medizinische Fakultät (Charité), Humboldt-Universität zu Berlin, Deutschland.
'In-line filtration' was performed on whole blood (WB) of 12 healthy donors. The WB was drawn into the triple-bag Leukotrap-RC system. Six red cell concentrates (RCC) were filtrated 1 h after donation either with or without buffy-coat and stored 35 days in AS-3. We measured the loss of red cells, the residual white cell count (Nageotte chamber), platelet count in fresh frozen plasma (FFP); 2,3-DPG, K+, Hbe, pH, O2Hb and pO50. The system was easy to handle but needed circular centrifugation holders because the filter is placed and fixed just at the top of the bags during centrifugation. RCC without buffy coat led to a faster filtration (15 vs, 38 min) and to an improved FFP quality (platelet count 23 vs, 54 Gpt/l). The leucocyte removal rate of the integrated PALL-RC-350 was similar, residual white cell count less than 3 x 10(6), in both preparations. All storage parameters were found within the normal range except a significant increase of O2Hb in filtrated red cells compared with the unfiltered control.
'In-line filtration' was performed on whole blood (WB) of 12 healthy donors. The WB was drawn into the triple-bag Leukotrap-RC system. Six red cell concentrates (RCC) were filtrated 1 h after donation either with or without buffy-coat and stored 35 days in AS-3. We measured the loss of red cells, the residual white cell count (Nageotte chamber), platelet count in fresh frozen plasma (FFP); 2,3-DPG, K+, Hbe, pH, O2Hb and pO50. The system was easy to handle but needed circular centrifugation holders because the filter is placed and fixed just at the top of the bags during centrifugation. RCC without buffy coat led to a faster filtration (15 vs, 38 min) and to an improved FFP quality (platelet count 23 vs, 54 Gpt/l). The leucocyte removal rate of the integrated PALL-RC-350 was similar, residual white cell count less than 3 x 10(6), in both preparations. All storage parameters were found within the normal range except a significant increase of O2Hb in filtrated red cells compared with the unfiltered control.