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Related Experiment Videos

[Flow cytometry quality control in stem cell separation]

J Zingsem1, S Serke, V Weisbach

  • 1Abteilung für Transfusionsmedizin, Friedrich-Alexander-Universität Erlangen, Deutschland.

Beitrage Zur Infusionstherapie Und Transfusionsmedizin = Contributions to Infusion Therapy and Transfusion Medicine
|January 1, 1994
PubMed
Summary
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Daily monitoring of CD-34-positive cells in peripheral blood stem cell (PBSC) collections helps predict the number of colony-forming cells (CFC). This method optimizes PBSC separation for autologous transplantation, ensuring efficient stem cell collection.

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Transplantation Medicine

Context:

  • Peripheral blood stem cells (PBSC) are increasingly used for autologous transplantation as an alternative to bone marrow stem cells.
  • Accurate identification of optimal apheresis timing is crucial for successful PBSC collection.
  • Previous studies indicated that platelet (PLT) and white blood cell (WBC) counts are not reliable predictors of colony-forming cell (CFC) yield.

Purpose:

  • To establish a reliable method for determining the optimal timing for PBSC collection.
  • To assess the predictive value of CD-34-positive cell counts for CFC yield.
  • To optimize the efficiency and quality control of PBSC separation procedures.

Summary:

  • Daily monitoring of CD-34-positive cells in peripheral blood (PB) was implemented to identify the optimal initiation of PBSC collection.

Related Experiment Videos

  • CD-34+ cell counts in PBSC concentrates were assessed alongside routine cell counts and CFU testing.
  • A strong correlation was observed between CD-34+ cells and CFC, enabling prediction of CFC yield within 2 hours post-apheresis.

    • Impact:

    • CD-34+ cell enumeration provides a reliable method to predict CFC yield, improving the efficiency of PBSC collection.
    • This approach allows for precise determination of the number of apheresis procedures required per patient.
    • Enhanced quality control and predictability in PBSC separation contribute to more successful autologous transplantation outcomes.