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[Preparation of leukocyte-reduced platelet concentrates from buffy coats using a novel separation apparatus]
Abstract:
A newly designed equipment of producing white cell-reduced platelet concentrates (PC) from buffy coat (BC) will be briefly described. A special insert and bag holder for centrifugation including a modified expressor (Patent 4205712) for careful drainage of the PC into the transfer bag from BC was developed. PCs prepared with this technique had an average volume of 70.60 +/- 6.44 ml, platelet content of (0.75 +/- 18) x 10(11), WBC content of (0.32 +/- 0.24) x 10(6) per unit, and 4.41 +/- 3.68 WBC/microliter.