Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-throughput genetic analysis using microfabricated 96-sample capillary array electrophoresis microplates

P C Simpson1, D Roach, A T Woolley

  • 1Department of Chemistry, College of Chemistry, University of California, Berkeley, CA 94720, USA.

Proceedings of the National Academy of Sciences of the United States of America
|April 16, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chronic therapy with α1A-adrenergic agonist reverses RV failure and mitochondrial dysfunction.

bioRxiv : the preprint server for biology·2026
Same author

Black Hole Spectroscopy and Tests of General Relativity with GW250114.

Physical review letters·2026
Same author

2025 British Society for Haematology Guideline for the treatment of chronic lymphocytic leukaemia.

British journal of haematology·2025
Same author

GW250114: Testing Hawking's Area Law and the Kerr Nature of Black Holes.

Physical review letters·2025
Same author

Erratum: Centrality-Dependent Modification of Jet-Production Rates in Deuteron-Gold Collisions at sqrt[s_{NN}]=200  GeV [Phys. Rev. Lett. 116, 122301 (2016)].

Physical review letters·2025
Same author

First Measurement of Hard Exclusive π^{-}Δ^{++} Electroproduction Beam-Spin Asymmetries off the Proton.

Physical review letters·2023
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

New capillary array electrophoresis (CAE) microplates enable high-throughput genetic analysis. These microplates analyze 96 samples in under 8 minutes, offering a 50-100x speed increase over traditional methods.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Genomics

Background:

  • Conventional slab gel electrophoresis is time-consuming for high-throughput genetic analysis.
  • There is a need for faster, more efficient methods for analyzing large sample sets.

Purpose of the Study:

  • To develop and demonstrate the functionality of novel capillary array electrophoresis (CAE) microplates for rapid genetic analysis.
  • To achieve high-throughput sample processing for genetic diagnostics.

Main Methods:

  • Fabrication of CAE microplates using micromachined glass wafers bonded into a sandwich structure.
  • Integration of 96 sample wells, 48 separation channels, an injection unit, and an electrode array.
  • Utilizing a laser-excited confocal fluorescence scanner for high temporal resolution detection.

Related Experiment Videos

Main Results:

  • Successful production of CAE microplates capable of analyzing 96 samples in under 8 minutes.
  • Demonstrated electrophoretic separation and fluorescence detection of a marker for hereditary hemochromatosis diagnosis.
  • Achieved a throughput 50 to 100 times greater than conventional slab gels.

Conclusions:

  • CAE microplates offer a significant advancement in speed and efficiency for genetic analysis.
  • These microplates are suitable for various high-throughput genetic applications, including diagnostics.
  • The developed technology facilitates rapid and comprehensive genetic screening.