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Potential pro-inflammatory effects of soluble E-selectin upon neutrophil function
M H Ruchaud-Sparagano1, E M Drost, S C Donnelly
1Respiratory Medicine Unit, University of Edinburgh Medical School, GB.
European Journal of Immunology
|March 4, 1998
Summary
Elevated soluble E-selectin in patients with acute respiratory disease syndrome (ARDS) potentiates neutrophil functions, increasing inflammation. This study reveals soluble E-selectin
Area of Science:
- Immunology
- Cell Biology
- Pulmonary Medicine
Background:
- Neutrophil recruitment is crucial for inflammatory responses to infection and injury.
- Selectins play a role in neutrophil adhesion and leukocyte recruitment.
- Elevated soluble E-selectin is observed in established acute respiratory disease syndrome (ARDS).
Purpose of the Study:
- To investigate the link between elevated soluble E-selectin and ARDS progression.
- To examine the effects of soluble recombinant E-selectin (E-zz) on neutrophil function.
Main Methods:
- Assessed plasma levels of soluble E-selectin in ARDS patients.
- Exposed neutrophils to soluble recombinant E-selectin (E-zz).
- Measured neutrophil polarization, beta2 integrin-mediated adhesion, surface expression, and chemotaxis.
Main Results:
- Soluble E-selectin exposure potentiated neutrophil polarization and beta2 integrin-mediated adhesion.
- Neutrophil deformability was unaffected by E-zz.
- E-zz inhibited chemotaxis but augmented reactive oxidant species production and superoxide anion release.
Conclusions:
- Soluble E-selectin may exert pro-inflammatory effects on neutrophils.
- These effects could exacerbate disease processes in ARDS.
- Soluble E-selectin represents a potential therapeutic target in ARDS.