Jove
Visualize
Contact Us

Related Experiment Videos

Slipped loop structure of DNA: a specific nucleotide sequence forms only one unique conformer

E B Khomyakova1, M V Petrova, E E Minyat

  • 1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.

FEBS Letters
|March 7, 1998
PubMed
Summary

Researchers confirmed a unique slipped loop structure (SLS) in DNA, stabilizing it with an interloop helix. This finding resolves previous ambiguities about potential isomers in polynucleotide chain folding.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-Invasive Measurement of Brain Temperature using Microwave Radiothermometry.

Bulletin of experimental biology and medicine·2026
Same author

[Effect of therapeutic hypothermia on post-stroke sleep disorders].

Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·2025
Same author

[Craniocerebral hypothermia in the acute period of ischemic stroke].

Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·2023
Same author

Chronic Critical Illness: Current Aspects of the Problem (Review).

Sovremennye tekhnologii v meditsine·2023
Same author

[Two models of insulin resistance development and the strategy to combat age-related diseases: literature review].

Problemy endokrinologii·2022
Same author

Thermal Balance of the Brain and Markers of Inflammatory Response in Patients with Schizophrenia.

Bulletin of experimental biology and medicine·2022
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • Previous studies demonstrated the existence of a novel polynucleotide chain folding termed slipped loop structure (SLS).
  • The possibility of two distinct SLS isomers existing in equilibrium remained experimentally unconfirmed.

Purpose of the Study:

  • To design and investigate a DNA structure that favors a unique slipped loop conformation.
  • To stabilize the specific conformation and rule out the presence of alternative isomers.

Main Methods:

  • Design of a bimolecular structure (SLS31) with two distamycin A binding sites.
  • Chemical probing of the DNA structure in the presence and absence of distamycin A.
  • Mapping of accessible nucleotides to determine structural conformation.

Related Experiment Videos

Main Results:

  • Experimental data did not indicate the presence of two isomers with distinct loop slippage.
  • The results strongly support the formation of a single, stable slipped loop conformation.

Conclusions:

  • The designed oligonucleotide structure forms a unique slipped loop conformation.
  • An additional interloop helix stabilizes this unique slipped loop structure, confirming its existence.