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Refinement of pepsin digestion method for isolation of Toxoplasma gondii from infected tissues

J P Dubey1

  • 1United States Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Parasite Biology and Epidemiology Lab., Beltsville, MD 20705-2350, USA. jdubey@ggpl.arsusda.gov

Veterinary Parasitology
|March 11, 1998
PubMed

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Toxoplasmosis, a zoonotic disease caused by the protozoan Toxoplasma gondii, poses significant public health challenges globally due to its high seroprevalence and varied clinical manifestations. As an obligate intracellular parasite, T. gondii can infect all warm-blooded vertebrates, but felids are its only definitive hosts, shedding unsporulated oocysts into the environment. Humans typically acquire the infection through ingestion of tissue cysts in undercooked meat or oocysts from...