Sensitive method for the detection of fumonisin B1 in human urine
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1Food and Drug Toxicology Research Centre, National Institute of Nutrition, Indian Council of Medical Research, Hyderabad.
Abstract:
Fumonisins are a group of carcinogenic mycotoxins that occur worldwide. This paper reports a sensitive method for the detection and quantitation of fumonisin B1 in human urine. Amberlite XAD-2 non-ionic polymeric adsorbent resin is used prior to strong anion-exchange (SAX) cartridge clean-up. As much as 100 ml of undiluted human urine can be loaded onto the column. Recoveries obtained using this method were 93.6, 95.1 and 94.4% when samples were spiked with 10, 50 and 500 ng/ml of fumonisin B1, respectively. This method is highly reproducible (R.S.D.<5%) and gives good sample clean-up, which is suitable for HPLC analysis.
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