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Updated: Aug 9, 2026

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
Published on: April 23, 2012
Sensitive method for the detection of fumonisin B1 in human urine
1Food and Drug Toxicology Research Centre, National Institute of Nutrition, Indian Council of Medical Research, Hyderabad.
Abstract:
Fumonisins are a group of carcinogenic mycotoxins that occur worldwide. This paper reports a sensitive method for the detection and quantitation of fumonisin B1 in human urine. Amberlite XAD-2 non-ionic polymeric adsorbent resin is used prior to strong anion-exchange (SAX) cartridge clean-up. As much as 100 ml of undiluted human urine can be loaded onto the column. Recoveries obtained using this method were 93.6, 95.1 and 94.4% when samples were spiked with 10, 50 and 500 ng/ml of fumonisin B1, respectively. This method is highly reproducible (R.S.D.<5%) and gives good sample clean-up, which is suitable for HPLC analysis.
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