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CD38 functions are regulated through an internalization step

A Funaro1, M Reinis, O Trubiani

  • 1Department of Genetics, Biology and Biochemistry, University of Torino, Turin, Italy.

Journal of Immunology (Baltimore, Md. : 1950)
|March 14, 1998
PubMed
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The human CD38 molecule undergoes endocytosis upon binding antibodies, independent of signal transduction. This process may regulate cell activation and adhesion, acting as a negative feedback mechanism.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • The CD38 molecule plays roles in cell activation and adhesion.
  • Understanding CD38's internalization is crucial for its functional characterization.

Purpose of the Study:

  • To investigate the endocytosis of the human CD38 molecule in lymphocytes and leukemia/lymphoma cell lines.
  • To determine the mechanisms and functional implications of CD38 internalization.

Main Methods:

  • Radioiodinated antibodies and acidic elution assay to quantify CD38 internalization.
  • Conventional, confocal, and electron microscopy to observe morphologic changes.
  • Investigated effects of both agonistic and nonagonistic antibodies.

Main Results:

  • CD38 internalization is a reproducible process following antibody ligation, affecting only a fraction of surface molecules.

Related Experiment Videos

  • Internalization is independent of signal transduction, occurring at a slower rate than cellular signaling.
  • Morphologic studies revealed a specific endocytic pathway for CD38.

    • Conclusions:

    • CD38 down-regulation via endocytosis, alongside shedding, may regulate CD38-mediated activation and adhesion.
    • Internalization appears to be a negative feedback mechanism interrupting signal transduction rather than triggering it.