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Posterior vitreous detachment with dispase

T H Tezel1, L V Del Priore, H J Kaplan

  • 1Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, Missouri 63110, USA.

Retina (Philadelphia, Pa.)
|March 21, 1998
PubMed
Summary
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Dispase effectively induces posterior vitreous detachment (PVD) in both porcine and human eyes in vitro. This enzyme cleaves the vitreous-retinal interface with minimal retinal damage, suggesting its potential use in vitreous surgery.

Area of Science:

  • Ophthalmology
  • Biochemistry
  • Cell Biology

Background:

  • Posterior vitreous detachment (PVD) is a common age-related condition.
  • Understanding the mechanisms and potential interventions for PVD is crucial for treating associated retinal pathologies.

Purpose of the Study:

  • To investigate the efficacy of Dispase in inducing PVD in ex vivo porcine and human eyes.
  • To evaluate the safety and structural integrity of the retina following Dispase treatment.

Main Methods:

  • Enucleated porcine and human eyes were treated with varying concentrations of Dispase.
  • Incubation times ranged from 15 to 120 minutes.
  • PVD induction was graded, and retinal structural integrity, cell viability, and ultrastructure were assessed via mechanical testing and microscopy.

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Main Results:

  • Dispase induced PVD in a dose- and time-dependent manner in porcine eyes.
  • A high success rate of complete PVD was observed in human cadaver eyes.
  • Microscopic analysis revealed Dispase cleaved the vitreous-retinal adhesion with minimal impact on retinal cell viability and structure.

Conclusions:

  • Dispase effectively disrupts the posterior hyaloid-internal limiting membrane attachment.
  • The enzyme demonstrates a favorable safety profile with minimal observed retinal damage.
  • Dispase shows promise as a tool for cortical vitreous removal in vitreous surgery.