Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The pre-UV nutritional stresses increase UV resistance, decrease UV mutagenesis and inhibit excision repair

V Slezarikova1, F Masek, M Pirsel

  • 1Department of Molecular Genetics, Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovak Republic.

Mutation Research
|March 20, 1998
PubMed
Summary

Nutritional stresses enhance E. coli UV resistance by inhibiting pyrimidine dimer excision (PDE). This uvrA-dependent mechanism promotes efficient, precise lesion tolerance, offering new insights into bacterial DNA repair strategies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcription factor IIH - the protein complex with multiple functions.

Neoplasma·2010
Same author

Involvement of the nucleotide excision repair proteins in the removal of oxidative DNA base damage in mammalian cells.

Neoplasma·2003
Same author

Role of DNA polymerase II in the tolerance of thymine dimers remaining unexcised in UV-irradiated Escherichia coli exposed to pre-UV nutritional stress.

Journal of photochemistry and photobiology. B, Biology·2002
Same author

Approaches to identification of HNPCC suspected patients in Slovak population.

Neoplasma·2000
Same author

Inducible stable DNA replication (iSDR) and the uvr-dependent tolerance of pyrimidine dimers in UV-irradiated Escherichia coli are two uncoupled processes.

Journal of photochemistry and photobiology. B, Biology·2000
Same author

Lack of correlation between repair of DNA interstrand cross-links and differential sensitivity of G0 and proliferating CD4+ lymphocytes towards cisplatin.

Neoplasma·2000

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Nutritional stresses in E. coli enhance resistance to UV irradiation and reduce mutagenesis.
  • This phenomenon is linked to the uvrA gene and potentially more efficient pyrimidine dimer excision.

Purpose of the Study:

  • To investigate the effect of nutritional stresses (glucose or amino acid starvation) on pyrimidine dimer excision (PDE) in E. coli.
  • To explore the mechanisms underlying stress-induced UV tolerance.

Main Methods:

  • E. coli cultures were subjected to prestarvation conditions (glucose or amino acids).
  • UV irradiation was applied, and subsequent DNA repair mechanisms, specifically PDE, were analyzed.
  • The role of the uvrA gene in stress-induced tolerance was examined.

Related Experiment Videos

Main Results:

  • Prestarvation for glucose or amino acids partially inhibited pyrimidine dimer excision (PDE).
  • Despite inhibited PDE, E. coli exhibited increased UV resistance.
  • A uvrA-dependent mode of lesion tolerance was identified, which is efficient and precise.

Conclusions:

  • Nutritional stress inhibits PDE but induces an efficient and precise uvrA-dependent tolerance mechanism against UV-induced DNA damage.
  • The study suggests a complex interplay between nutritional status, DNA repair pathways, and bacterial survival under stress.
  • Further research is warranted to elucidate the precise molecular mechanisms of PDE inhibition and lesion tolerance.