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Practical approach for detection and identification of OXA-10-derived ceftazidime-hydrolyzing extended-spectrum
H Vahaboglu1, R Ozturk, H Akbal
1Kocaeli Universitesi, Tip Fakültesi, Turkey. vahabo@uedh.com.tr
Journal of Clinical Microbiology
|March 21, 1998
Abstract:
A practical approach to detect and identify ceftazidime-hydrolyzing extended-spectrum mutants of OXA-10 beta-lactamase is presented. Large numbers of bacteria were screened by colony hybridization, a 720-bp part of blaOXA was amplified by PCR from the hybridization-positive isolates, and the products were digested by PvuII and HaeIII.