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Related Experiment Videos

Protein adsorption onto ceramic surfaces

Y Takami1, S Yamane, K Makinouchi

  • 1Baylor College of Medicine, Department of Surgery, Houston, Texas 77030, USA.

Journal of Biomedical Materials Research
|March 25, 1998
PubMed
Summary
This summary is machine-generated.

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Ceramics like alumina (Al2O3) and silicon carbide (SiC) show reduced protein adsorption compared to plastics. This suggests ceramics are thromboresistant materials for blood-contacting devices.

Area of Science:

  • Biomaterials Science
  • Medical Device Engineering
  • Surface Chemistry

Background:

  • Ceramics are rarely used in blood-contacting applications.
  • Alumina ceramic (Al2O3) and polyethylene are used in Gyro centrifugal blood pump pivot bearings due to durability.
  • Pivot bearing systems are critical in centrifugal pumps due to potential stagnant flow, impacting hemocompatibility.

Purpose of the Study:

  • To evaluate the thrombogenicity of pivot bearings in the Gyro pump.
  • To investigate protein adsorption, specifically albumin, IgG, fibrinogen, and fibronectin, onto ceramic surfaces.
  • To compare alumina ceramic (Al2O3) and silicon carbide ceramic (SiC) with polyethylene (PE) and polyvinylchloride (PVC).

Main Methods:

  • Protein adsorption assays using Bicinchoninic acid (BCA) protein assay.

Related Experiment Videos

  • Analysis of adsorbed proteins via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
  • Identification of specific adsorbed proteins using Western immunoblotting.

    • Main Results:

    • Al2O3 and SiC exhibited significantly less overall protein adsorption than PVC.
    • SDS-PAGE showed fewer proteins adsorbed onto Al2O3 and SiC compared to PVC and PE.
    • Western immunoblotting revealed similar albumin adsorption across all materials, but lower IgG, fibrinogen, and fibronectin adsorption on Al2O3 and SiC versus PE and PVC.

    Conclusions:

    • Ceramics (Al2O3 and SiC) demonstrate reduced protein adsorption.
    • Lower protein adsorption suggests potential thromboresistance for ceramic materials in blood-contacting applications.
    • Ceramics are promising candidates for developing more hemocompatible blood pump components.