Related Experiment Video

Updated: Jul 28, 2026

The Portable Chemical Sterilizer (PCS), D-FENS, and D-FEND ALL: Novel Chlorine Dioxide Decontamination Technologies for the Military
14:17

The Portable Chemical Sterilizer (PCS), D-FENS, and D-FEND ALL: Novel Chlorine Dioxide Decontamination Technologies for the Military

Published on: June 29, 2014

[The microorganism count on impressions after their disinfection by submersion in sodium hypochlorite solutions]

T N Koshmanova, L G Panteleeva

    Stomatologiia
    |March 25, 1998
    PubMed

    Insights

    Sodium hypochlorite (0.5%) effectively inactivates poliomyelitis virus, bacteriophage, Staphylococcus aureus, and Candida albicans within 20 minutes. This disinfectant also destroys silicone and alginate imprints, demonstrating broad antimicrobial and material compatibility.

    Area of Science:

    • Microbiology
    • Infectious Diseases
    • Materials Science

    Background:

    • Sodium hypochlorite is a widely used disinfectant.
    • Its efficacy against various microorganisms and materials needs thorough evaluation.
    • Understanding its interaction with imprint materials is crucial for disinfection protocols.

    Purpose of the Study:

    • To assess the virucidal, bactericidal, and fungicidal activity of sodium hypochlorite.
    • To determine the effect of sodium hypochlorite on silicone and alginate imprint materials.
    • To establish effective disinfection parameters for common pathogens and dental materials.

    Main Methods:

    • Testing the disinfectant activity against Poliomyelitis virus, bacteriophage f52, Staphylococcus aureus, and Candida albicans.
    • Submerging silicone and alginate imprints in 0.5% sodium hypochlorite solution.
    • Quantifying microbial inactivation and material degradation over a 20-minute period.

    Main Results:

    • 0.5% sodium hypochlorite completely inactivated all tested microorganisms (viruses, bacteria, fungi) within 20 minutes, even in the presence of protein.
    • Silicone imprints showed complete microbial inactivation.
    • Alginate imprints were destroyed upon submersion in the sodium hypochlorite solution.

    Conclusions:

    • Sodium hypochlorite exhibits potent broad-spectrum antimicrobial activity.
    • The disinfectant is effective for sterilizing silicone imprints.
    • Care must be taken when using sodium hypochlorite with alginate materials due to potential degradation.

    More Related Videos

    A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens09:02

    A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens

    Published on: June 7, 2017

    Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods11:47

    Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

    Published on: December 9, 2022

    Related Concept Videos

    Hand hygiene01:23

    Hand hygiene

    Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
    Hand washing...
    Cleaning, Sterilization, and Disinfection01:30

    Cleaning, Sterilization, and Disinfection

    Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
    Cleaning
    The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
    Methods of Sterilization II: Chemical Methods01:30

    Methods of Sterilization II: Chemical Methods

    In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
    Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
    Chemical Agents for Microbial Control01:27

    Chemical Agents for Microbial Control

    Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
    Antimicrobial Effectiveness01:28

    Antimicrobial Effectiveness

    The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
    Microbial Bioremediation of Pesticides01:28

    Microbial Bioremediation of Pesticides

    Pesticides often feature structurally complex chemical architectures, incorporating halogen groups and multiple aromatic rings. These characteristics confer high chemical stability, rendering many pesticides resistant to natural degradation processes. This resistance poses significant environmental concerns, as persistent pesticide residues can accumulate in ecosystems and affect non-target organisms.Despite the inherent stability of many pesticides, certain microorganisms possess the metabolic...