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HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL
Published on: April 11, 2011
Major histocompatibility complex class II-dependent antigen presentation by human intestinal endothelial cells
G Haraldsen1, L M Sollid, O Bakke
1Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), Institute of Pathology, University of Oslo, Rikhospitalet, Oslo, Norway. guttormh@cmgm.stanford.edu
Gastroenterology
|April 18, 1998
Summary
Human intestinal microvascular endothelial cells (HIMECs) can present complex protein antigens to T cells. Cytokines like interferon-gamma induce major histocompatibility complex (MHC) class II expression in HIMECs, enabling antigen presentation in the gut.
Area of Science:
- Immunology
- Gastroenterology
- Cell Biology
Background:
- Human intestinal microvascular endothelial cells (HIMECs) normally express major histocompatibility complex (MHC) class II molecules.
- Expression of MHC class II is enhanced in chronic gut inflammation.
Purpose of the Study:
- To examine cytokine regulation of MHC class II molecules and invariant chain (Ii) in HIMECs.
- To investigate if HIMECs can process and present complex protein antigens to T cells.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA)
- Flow cytometry
- Immunoelectron microscopy
- T-cell activation assays with HIMECs and HLA-DR-restricted T-cell clones
Main Results:
- Interferon-gamma (IFN-γ) induced MHC class II (HLA-DR, -DP, -DQ) and invariant chain (Ii) expression in HIMECs.
- Tumor necrosis factor alpha enhanced IFN-γ-induced class II expression.
- IFN-γ-treated HIMECs activated T-cell clones in response to Mycobacterium tuberculosis antigen, demonstrating antigen presentation.
Conclusions:
- Microvascular endothelial cells in the human gut possess the capability to present complex protein antigens.
- This finding has implications for understanding immune responses within the gut microvasculature.
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