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Related Experiment Videos

Fluorescence analysis of cells using a laser light source

M W Berns

    Cell Biophysics
    |March 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    A new quantitative microfluorometric instrument uses a helium cadmium laser for precise nucleic acid measurements. This advanced system accurately determines cell ploidy levels, offering superior fluorescence compared to standard lamps.

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    Area of Science:

    • Biophysics
    • Cell Biology
    • Instrumentation

    Background:

    • Quantitative microfluorometry is essential for cellular analysis.
    • Existing instrumentation may have limitations in sensitivity and accuracy.
    • Nucleic acid quantification requires precise fluorescence measurement.

    Purpose of the Study:

    • To describe a novel quantitative microfluorometric instrument.
    • To demonstrate its capability for nucleic acid measurements.
    • To evaluate its performance in determining cell ploidy levels.

    Main Methods:

    • Utilized a helium cadmium laser (442 nm) as the illumination source.
    • Incorporated a double grating monochromator and a gallium arsenide photomultiplier.
    • Interfaced the system with a desktop computer for data acquisition and analysis.

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  • Employed acridine orange and Feulgen-Schiff staining for nucleic acid detection.

    • Main Results:

    • Successfully demonstrated quantitative nucleic acid measurements in stained cells.
    • Accurately determined ploidy levels of several cell populations.
    • Achieved significantly greater Feulgen fluorescent emissions compared to standard mercury lamps.

    Conclusions:

    • The developed instrument offers a versatile and sensitive platform for quantitative microfluorometry.
    • It provides accurate cell ploidy analysis with enhanced fluorescence detection.
    • This technology advances nucleic acid quantification in biological research.