Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Origin DNA-binding proteins

A M Edwards1, A Bochkarev, L Frappier

  • 1Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada. aled.edwards@utoronto.ca

Current Opinion in Structural Biology
|March 31, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cancer blues? A promising judgment bias task indicates pessimism in nude mice with tumors.

Physiology & behavior·2021
Same author

Maternal effects obscure condition-dependent sex allocation in changing environments.

Royal Society open science·2019
Same author

Notes on Cases of Injury to the Skull and Its Contents.

Glasgow medical journal·2018
Same author

Blood pressure, arterial stiffness and exercise: does exercise increase the risk of acute cardiac events in older adults?

European journal of preventive cardiology·2018
Same author

Remarks on Sayre's Splint for Morbus Coxarius.

Edinburgh medical journal·2018
Same author

Notes on the State of Lithotomy, past and Present. From a Lecture.

Edinburgh medical journal·2018
Same journal

Tomogram exploration through template matching and deep learning.

Current opinion in structural biology·2026
Same journal

A comparative review of cryo-electron ptychography: Biological applications and future perspectives.

Current opinion in structural biology·2026
Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026
Same journal

Collective variable design for biomolecular conformational dynamics.

Current opinion in structural biology·2026
Same journal

Polymer scaling in protein crowding: From dilute coils to semidilute meshes.

Current opinion in structural biology·2026
Same journal

Tuning the physicochemical properties of rationally designed protein-based biomolecular condensates.

Current opinion in structural biology·2026
See all related articles

DNA replication initiation involves origin-binding proteins recognizing specific DNA sequences. Three distinct origin-binding protein structures reveal a unique structural class, with Epstein-Barr virus nuclear antigen 1 potentially using dual binding modes for replication initiation.

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Virology

Background:

  • DNA replication is a fundamental biological process initiated at specific DNA sequences.
  • Origin-binding proteins play a crucial role in recognizing and binding to these origin DNA sequences.
  • Understanding the structure of these proteins is key to elucidating the mechanism of replication initiation.

Purpose of the Study:

  • To describe the structural characteristics of origin DNA-binding proteins.
  • To investigate the structural class of these proteins.
  • To explore the potential DNA binding modes of Epstein-Barr virus nuclear antigen 1.

Main Methods:

  • X-ray crystallography or cryo-electron microscopy for structure determination.
  • Comparative structural analysis of different origin-binding proteins.

Related Experiment Videos

  • Bioinformatic analysis of protein structures and DNA interaction interfaces.

    • Main Results:

    • The three-dimensional structures of three distinct origin DNA-binding proteins were determined.
    • These proteins belong to a unique structural class, differing from other known DNA-binding proteins.
    • Epstein-Barr virus nuclear antigen 1 exhibits characteristics suggesting two potential DNA binding modes.

    Conclusions:

    • Origin-binding proteins possess a distinct structural fold.
    • The structural diversity of origin-binding proteins is limited.
    • Epstein-Barr virus nuclear antigen 1's dual DNA binding modes may be critical for initiating DNA replication.