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Isolation and characterization of the dnaKJ operon from Actinobacillus actinomycetemcomitans
A Yoshida1, Y Nakano, Y Yamashita
1First Department of Oral and Maxillofacial Surgery, Kyushu University Faculty of Dentistry, Fukuoka, Japan.
DNA Sequence : the Journal of DNA Sequencing and Mapping
|January 1, 1997
Abstract:
The dnaKJ operon of Actinobacillus actinomycetemcomitans Y4 was cloned by the DNA-probing method using synthetic oligonucleotides designed on the basis of two conserved regions in DnaK/hsp70 proteins and sequenced by inverse PCR. The sequenced region was shown to contain two open reading frames coding for proteins analogous to DnaK and DnaJ.