Nucleotide and amino acid sequences of the monkey P450 2B gene subfamily

The nucleotide sequence of a cDNA coding for monkey cytochrome P450 (P450) 2B has been determined. Using antibody against P450 CMLa which had been purified from hepatic microsomes of untreated cynomolgus monkeys, a cDNA clone with 2,275 bp insert (Mac2B) was isolated from a gamma gt11 cDNA library prepared from hepatic mRNA from an untreated rhesus monkey. The cloned insert was sequenced and found to contain an open reading frame coding for a polypeptide of 491 amino acids. The molecular weight calculated from the deduced amino acid sequence was 55,969. The N-terminal 34 amino acids encoded by Mac2B were identical to those determined by Edman degradation analysis of purified cynomolgus monkey P450 CMLa. The nucleotide and the deduced amino acid sequences of Mac2B which is now called CYP2B17 were the most similar to those of human P450 2B6 among the P450 2B subfamily and those sequences of Mac2B were 94% and 90% identical to those of human P450 2B6, respectively.