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Isolation and characterization of site-specific endonuclease Bsp123 I from thermophilic strain Bacillus species 123
D V Alzhanova1, D T Alzhanov, L A Zheleznaya
1Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia. nikmatv@sun.ipr.serpukhov.su
Biochemistry. Biokhimiia
|June 2, 1998
Abstract:
The preparation of site-specific endonuclease Bsp123 I was isolated and purified from the thermophilic strain Bacillus species 123. Endonuclease Bsp123 I recognizes the sequence CGCG and cleaves it in the middle between nucleotides G and C, producing blunt ends. Thus, Bsp123 I is an isoschizomer of FnuDII. The maximal activity of the enzyme is displayed at 42 degrees C, pH 8.0, 100 mM NaCl, 10 mM MgCl2.