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[Sequencing analysis of human K-ras oncogene exon 1 in Chinese people]
1Laboratory of Molecular Genetics, The First People's Public Hospital in Huaihua, Hunan 418000 P. R. China.
Abstract
Objective:
To investigate the mutation of human K-ras oncogene in the blood of patients with lung cancer.
Methods:
PCR and sequencing methods were used to analyse the exon 1. Twelve patients'blood samples and 31 normals' blood samples were detected.
Results:
In all 43 samples, the K-ras codon 11 was CCT (proline), and the opposite strand sequenced was AGG. It was different from the standard GCT(Alanine) sequenced by foreign authors.
Conclusion:
The 11th codon of the human K-ras oncogene in Chinese is CCT (Proline). As the K-ras codon 12 is a hot spot, this finding may be of importance to clinical genetic diagnosis.