[Gas chromatography of organic acids: methodological and diagnostic aspects]

Gas liquid chromatography (GLC) remains the most efficient method for analysis of organic acids in biological fluids. This paper presents a GLC method for measuring organic acids in the urine. Our method of extraction and GLC of trimethylsilyl derivatives of organic acids is a specific and highly sensitive procedure which permits investigation of the complex spectrum of organic acids, including aliphatic oxocarbonic, dicarbonic, hydroxycarbonic, and aromatic acids and glycine conjugates in the urine and other physiological fluids. It can be used for the diagnosis and prenatal screening of hereditary and acquired metabolic disorders.