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Detection of goose and Muscovy duck parvoviruses using polymerase chain reaction-restriction enzyme fragment length
P Sirivan1, M Obayashi, M Nakamura
1National Institute of Animal Health, Chatuchak, Bangkok, Thailand.
Abstract:
By using primers (AL18F2 and AL18R2) designed from goose parvovirus (GPV) strain IHC, an 806-bp band was amplified by polymerase chain reaction (PCR) from all of 17 samples from Thailand. Specificity to GPV was confirmed by Southern hybridization. With restriction enzyme digestion of the PCR products, two isolates differed from the other 15 isolates by the absence of restriction sites for HincII and BglII and the presence of EcoR1 site. Nucleotide sequence analysis of the PCR products from the different groups revealed that one group is GPV and the other group is Muscovy duck parvovirus (MDPV). Thus restriction enzyme fragment length polymorphism analysis of the PCR products could be used to distinguish GPV and MDPV. The data showed that GPV and MDPV are present in Thailand.