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Effect of glucan on murine lungs
1Albuquerque VA Medical Center, University of New Mexico School of Medicine, 87108, USA.
Journal of Toxicology and Environmental Health. Part A
|April 16, 1998
Summary
Intratracheal glucan administration causes dose-dependent lung injury in animals. However, this pulmonary inflammation is not immunologically mediated by cell transfer, despite glucan exposure inducing lymphoblast-like cells in culture.
Area of Science:
- Immunology
- Pulmonary Medicine
- Toxicology
Background:
- Glucan, a high-molecular-weight polysaccharide, exhibits various effects in animal models when administered systemically.
- Previous studies indicate glucan's effects are route-dependent, with inhalation administration showing no significant impact.
Purpose of the Study:
- To investigate whether intratracheal administration of glucan induces lung damage.
- To determine if glucan-induced lung injury involves an immunological, cell-mediated response.
Main Methods:
- Animals received varying doses of glucan via intratracheal injection.
- Histopathological analysis assessed pulmonary abnormalities and inflammation.
- An adoptive transfer model was employed, culturing cells with glucan and transferring them to naive recipients before glucan exposure.
Main Results:
- A clear dose-response relationship was observed between intratracheal glucan dose and the severity of pulmonary histologic abnormalities.
- Histologic findings included peribronchiolar and intraalveolar infiltration by chronic inflammatory cells.
- Adoptive transfer of cultured cells did not alter the extent of glucan-induced pulmonary inflammation in recipients.
- High glucan concentrations in vitro generated cells resembling lymphoblasts.
Conclusions:
- Intratracheal administration of glucan is capable of inducing significant lung injury in animals.
- The study found no evidence supporting a cell-mediated immunological mechanism for glucan-induced pulmonary injury following intratracheal exposure.