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Related Experiment Videos

Molecular coevolution within a Drosophila clock gene

A A Peixoto1, J M Hennessy, I Townson

  • 1Department of Genetics, University of Leicester, Leicester, LE1 7RH, United Kingdom.

Proceedings of the National Academy of Sciences of the United States of America
|May 16, 1998
PubMed
Summary
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The period gene

Area of Science:

  • Chronobiology
  • Molecular Biology
  • Genetics

Background:

  • The period (per) gene is crucial for biological rhythmicity in Drosophila melanogaster.
  • The PER protein contains a threonine-glycine (Thr-Gly) repeat, with similar repeats found in other species' clock genes.
  • Interspecies sequence comparisons suggest coevolution between the Thr-Gly repeat length and flanking amino acids in Drosophila.

Purpose of the Study:

  • To functionally test the hypothesis that the Thr-Gly repeat length and flanking amino acids in the Drosophila PER protein coevolve.
  • To investigate the functional significance of these coevolving regions for circadian rhythmicity and temperature compensation.

Main Methods:

  • Generation of several hybrid per transgenes by combining repetitive regions and flanking sequences from Drosophila melanogaster and Drosophila pseudoobscura.

Related Experiment Videos

  • Alteration of chimeric junction positions within the transgenes.
  • Functional analysis of transformants to assess circadian rhythmicity and temperature sensitivity.

    • Main Results:

    • Hybrid transgenes with species-specific repeat and flanking regions rescued circadian rhythmicity to wild-type levels.
    • Transformants with juxtaposed heterospecific repeat and flanking regions exhibited arrhythmicity or temperature-sensitive periods.
    • These findings indicate a functional link between the repeat length, flanking sequences, and circadian clock function.

    Conclusions:

    • The study supports the coevolutionary interpretation of sequence changes in the PER protein's repetitive region.
    • A functional dimension to this coevolution has been revealed, specifically its role in the circadian clock's temperature compensation.
    • The findings highlight the importance of conserved sequence elements and their interactions for maintaining circadian rhythmicity.