Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Comparative gene expression profiling by oligonucleotide fingerprinting

S Meier-Ewert1, J Lange, H Gerst

  • 1Max-Planck-Institut für Molekulare Genetik, Ihnestrasse 73, 14195 Berlin, Germany. sebastian.meier-ewert@gpc-ag.com

Nucleic Acids Research
|June 20, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond the LRINEC Score: A stratified diagnostic algorithm for subacute and atypical necrotizing fasciitis.

Annales de chirurgie plastique et esthetique·2026
Same author

The Absence of Claudin-10 in the Enamel Organ Alters Its Integrity.

Journal of dental research·2025
Same author

Primary central nervous system lymphoma of the spinal cord: A LOC network cohort study.

Revue neurologique·2024
Same author

CDK9 inhibition as an effective therapy for small cell lung cancer.

Cell death & disease·2024
Same author

Expanding self-consciousness of health status for diabetes chronic complications among adults: a systematic review.

European review for medical and pharmacological sciences·2024
Same author

Understanding morphological evolution and sediment dynamics at multi-time scales helps balance human activities and protect coastal ecosystems: An example with the Gironde and Pertuis Marine Park.

The Science of the total environment·2023
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
See all related articles

This study used cDNA fingerprinting to analyze gene expression in mouse embryos. The approach successfully identified gene clusters and differences in gene representation between developmental stages.

Area of Science:

  • Developmental Biology
  • Genomics
  • Molecular Biology

Background:

  • Characterizing gene sequences is crucial for understanding biological processes.
  • Hybridization techniques using synthetic oligonucleotides to cDNAs are established methods for gene sequence analysis.

Purpose of the Study:

  • To pilot a cDNA fingerprinting approach for characterizing expressed genes in mouse embryos.
  • To generate comparative gene expression profiles between different developmental stages.

Main Methods:

  • Utilized two cDNA libraries from 9 and 12-day mouse embryos.
  • Employed hybridization with 110 probes to analyze approximately 58,000 cDNA clones.
  • Applied statistical analysis to identify differentially represented gene clusters.

Related Experiment Videos

Main Results:

  • Identified 33,369 cDNA clone clusters.
  • Assigned 737 clusters to known rodent genes and found significant homology in 13,845 others.
  • Detected 404 clusters with significantly differential representation (P < 0.01) between the two embryonic stages.

Conclusions:

  • The cDNA fingerprinting method is effective for comparative gene expression profiling.
  • This approach facilitates the analysis of gene expression differences in distinct biological materials.
  • The study provides insights into gene expression dynamics during mouse embryonic development.