Related Experiment Video
Updated: Sep 24, 2026
Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
Validation of an LC-MS assay for the quantification of the enantiomers of Org 4428 in human plasma
J E Paanakker1, J de Jong, J M Thio
1Scientific Development Group, N.V. Organon, Department of Drug Metabolism and Kinetics, Oss, Netherlands.
Abstract:
A sensitive and selective liquid chromatographic mass spectrometric assay has been validated for the quantification of Org 4428 enantiomers in human plasma. The assay employs n-hexane extraction from alkalinized plasma, separation on a narrow-bore enantioselective normal phase Chiralpak AD column and APCI MS-MS detection. The lower limit of quantification is 0.5 ng ml-1 plasma for the individual enantiomers.
More Related Videos
06:04LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
Published on: April 4, 2016
08:56Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
Published on: November 22, 2024