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Related Experiment Video

Updated: Jul 24, 2026

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
07:30

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

Published on: September 4, 2017

Synaptic vesicles retain their identity through the endocytic cycle

V N Murthy1, C F Stevens

  • 1Howard Hughes Medical Institute and Molecular Neurobiology Laboratory, Salk Institute, La Jolla, California 92037, USA.

Nature
|April 21, 1998
PubMed
Summary

Synaptic vesicle recycling at central synapses bypasses endosome-like compartments. This study shows internalized vesicles directly reuse membrane, challenging the classical model of synaptic vesicle recycling.

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Area of Science:

  • Neuroscience
  • Cell Biology

Background:

  • Synaptic vesicles release neurotransmitters via exocytosis.
  • Vesicular membrane retrieval through endocytosis is crucial for synaptic function.
  • The precise recycling pathway of synaptic vesicles remains debated.

Purpose of the Study:

  • To investigate the endocytic recycling pathway of synaptic vesicles at central synapses.
  • To test the classical model of synaptic vesicle recycling involving endosomal intermediates.

Main Methods:

  • Utilized the fluorescent membrane dye FM1-43.
  • Employed quantitative fluorescence microscopy to track membrane dynamics.

Main Results:

  • The amount of FM1-43 dye taken up during endocytosis matched the amount released during exocytosis.

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An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins

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Live Imaging of Synaptic Vesicle Recycling in the Neuromuscular Junction of Dissected Larval Zebrafish
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Live Imaging of Synaptic Vesicle Recycling in the Neuromuscular Junction of Dissected Larval Zebrafish

Published on: February 7, 2025

Related Experiment Videos

Last Updated: Jul 24, 2026

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
07:30

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

Published on: September 4, 2017

An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins
09:33

An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins

Published on: June 26, 2018

Live Imaging of Synaptic Vesicle Recycling in the Neuromuscular Junction of Dissected Larval Zebrafish
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Live Imaging of Synaptic Vesicle Recycling in the Neuromuscular Junction of Dissected Larval Zebrafish

Published on: February 7, 2025

  • Internalized vesicles did not appear to interact with intermediate endosome-like compartments.

    • Conclusions:

    • Synaptic vesicles directly recycle their membrane without obligatory passage through endosomes.
    • The classical model of synaptic vesicle recycling requires revision for central synapses.