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CD14 expression and soluble CD14 after segmental allergen provocation in atopic asthma
J C Virchow1, P Julius, H Matthys
1Abteilung für Pneumologie, Robert-Koch-Klinik, Medizinische Universitätsklinik Freiburg, Germany.
The European Respiratory Journal
|April 29, 1998
Summary
Soluble CD14 (sCD14) levels increase 18 hours after allergen challenge in allergic asthma patients, suggesting CD14-mediated cell activation plays a role in airway inflammation.
Area of Science:
- Immunology
- Respiratory Medicine
- Cell Biology
Background:
- Allergic asthma involves inflammatory cell recruitment post-allergen exposure.
- CD14 surface expression is a potential marker for cell activation and differentiation.
Purpose of the Study:
- To investigate CD14 expression on activated immune cells and soluble CD14 (sCD14) levels in allergic asthma patients after allergen challenge.
- To explore the relationship between sCD14, inflammatory cells, and cytokines in the context of asthmatic inflammation.
Main Methods:
- Eight allergic asthma patients underwent segmental allergen provocation (SAP) and saline challenge.
- Flow cytometry measured CD14 expression on macrophages and granulocytes.
- ELISA analyzed sCD14 and interleukin (IL)-13 concentrations in blood and bronchoalveolar lavage (BAL) fluid at 10 minutes and 18 hours post-challenge.
Main Results:
- sCD14 levels significantly increased 18 hours after SAP, but not at 10 minutes or after saline challenge.
- Granulocyte numbers correlated with sCD14 levels 18 hours post-SAP, while their CD14 expression decreased.
- sCD14 concentrations correlated with IL-13 levels 18 hours after SAP.
Conclusions:
- The increase in sCD14 suggests local release of the CD14 antigen following allergen provocation in asthma.
- CD14-mediated cell activation may contribute to the inflammatory processes observed in allergic asthma.