Related Experiment Video
Updated: Aug 5, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Published on: June 6, 2017
Function and regulation of cdc25 protein phosphate through mitosis and meiosis
1Laboratoire de Physiologie de la Reproduction, INRA/URA-CNRS 1449, Université Pierre et Marie Curie, Paris, France.
Abstract:
Activation of the cyclin B-cdc2 kinase mitotic inducer involves dephosphorylation of two inhibitory residues, tyrosine 15 and threonine 14, cdc25 is the specific phosphatase that directly dephosphorylates and activates the cdc2 kinase, cdc25 activity is regulated by phosphorylation. Both phosphatases 1 and 2A could act as cdc25-specific inhibitory phosphatases. Although the cyclin B-cdc2 complex plays a role in activating cdc25, it is highly probable that a distinct protein kinase is involved as a trigger in cdc25 activation. The implication of raf kinase as a cdc25-specific activating kinase in human cells and Xenopus oocytes is discussed.
Related Concept Videos
Positive Regulator Molecules
Positive Regulator Molecules
Inhibition of Cdk Activity
M-Cdk Drives Transition Into Mitosis
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Separation of Sister Chromatids
At the onset of anaphase, separase, a proteolytic enzyme, is...
M-Cdk Drives Transition Into Mitosis
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

