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Ecto-ATPase: an activation marker necessary for effector cell function

K E Dombrowski1, Y Ke, K A Brewer

  • 1Atlanta Research and Education Foundation/Department of Veterans Affairs Medical Center, Decatur, Georgia 30033, USA. dombrowski.kenneth_e@atlanta.va.gov

Immunological Reviews
|April 29, 1998
PubMed
Summary
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Ecto-ATPase inhibition blocks immune cell functions like cytokine secretion and cytolytic activity. This enzyme

Area of Science:

  • Immunology
  • Cell Biology
  • Enzymology

Background:

  • Ecto-ATPase is a cell surface enzyme hydrolyzing extracellular ATP (ATPe).
  • It's expressed during immune cell activation.
  • Its role in lymphocyte effector functions is under investigation.

Purpose of the Study:

  • To investigate the role of ecto-ATPase in immune cell activation and effector functions.
  • To determine the effects of ecto-ATPase inhibition on T cells, B cells, and NK cells.

Main Methods:

  • Utilized non-hydrolyzable ATP analogues and 5'-p-(fluorosulfonyl)benzoyl adenosine (5'-FSBA) to inhibit ecto-ATPase.
  • Assessed antigen-induced cytokine secretion and cytolytic activity in T cells.
  • Measured granule release and cytolytic activity in NK cells.

Related Experiment Videos

  • Evaluated antibody secretion and proliferation in B-cell hybridomas.

    • Main Results:

    • Inhibition of ecto-ATPase by reversible and irreversible antagonists suppressed T cell cytokine secretion and cytolytic activity.
    • NK cell granule release and cytolytic activity were inhibited.
    • B cell antibody secretion and spontaneous proliferation were also reduced.
    • Ecto-ATPase inhibition did not affect effector cell-target cell conjugate formation.
    • Inhibition partially acts by regulating extracellular calcium influx necessary for cell activation.

    Conclusions:

    • Ecto-ATPase plays a crucial role in regulating lymphocyte effector functions.
    • Inhibiting ecto-ATPase impacts T cell, B cell, and NK cell activities.
    • The enzyme's regulation of calcium influx is key to its function in cellular activation.