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Expression of large genomic clones in transgenic mice: new insights into apolipoprotein B structure, function and
1Biochemistry Department, University of Otago, Dunedin, New Zealand. sally.mccormick@stonebow.otago.ac.nz
Abstract:
Extensive manipulation of the apolipoprotein B gene in yeast and bacterial artificial chromosome clones and subsequent expression of these clones in transgenic mice have provided fresh insights into several aspects of apolipoprotein B biology, including the identification of sequences important for lipoprotein (a) assembly, the demonstration that intestinal expression of apolipoprotein B is controlled by DNA sequences > 50 kb from the gene, and the extraordinary finding that apolipoprotein B is expressed in the heart.
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