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Published on: March 28, 2014
Sequestration of the G protein beta gamma subunit complex inhibits receptor-mediated endocytosis
H C Lin1, J A Duncan, T Kozasa
1Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75235-9041, USA.
Abstract:
Cell surface receptors that mediate endocytosis cluster into clathrin-coated pits, which pinch off to form vesicles that transport the receptors and their ligands. This multi-step process requires the coordinated action of many factors, including GTP-hydrolyzing proteins such as dynamin and regulators of actin cytoskeleton assembly. We note herein that sequestration of heterotrimeric G protein beta gamma subunits in intact cells strongly inhibits clathrin-coated pit-mediated endocytosis and causes rearrangement of the actin cytoskeleton. Our results suggest that cells contain a pool of free beta gamma and that it functions constitutively to permit endocytosis.
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