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Somatotropin-dependent decrease in fatty acid synthase mRNA abundance in 3T3-F442A adipocytes is the result of a

D Yin1, S D Clarke, J L Peters

  • 1Department of Dairy and Animal Science, The Pennsylvania State University, University Park, PA 16802, USA.

The Biochemical Journal
|April 30, 1998
PubMed
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Somatotropin (ST) significantly reduces fatty acid synthase (FAS) mRNA levels in adipocytes by decreasing both gene transcription and mRNA stability. This impacts lipogenesis and insulin

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Insulin stimulates lipogenesis and fatty acid synthase (FAS) mRNA abundance in adipocytes.
  • Somatotropin (ST) is known to decrease lipogenesis and FAS enzyme activity.

Purpose of the Study:

  • To investigate the molecular mechanisms by which ST reduces FAS mRNA levels in 3T3-F442A adipocytes.
  • To determine if ST affects FAS gene transcription and/or FAS mRNA stability.

Main Methods:

  • Run-on analyses to assess gene transcription rates.
  • Measurement of FAS mRNA half-life.
  • Stable transfection of 3T3-F442A cells with a FAS gene reporter construct (pFAS-CAT5).

Main Results:

Related Experiment Videos

  • ST decreased FAS mRNA levels by reducing transcription by 82% and shortening mRNA half-life from 35 to 11 hours.
  • ST significantly reduced the stimulatory effects of insulin on FAS mRNA abundance and lipogenesis.
  • Reporter gene assays confirmed that ST markedly reduced the transcriptional activity of the FAS gene.

    • Conclusions:

    • ST decreases adipocyte lipogenesis by reducing both the transcription of the FAS gene and the stability of FAS mRNA.
    • ST counteracts the lipogenic effects of insulin at the level of FAS gene expression.