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Molecular characterization of metal-binding polypeptide domains by electrospray ionization mass spectrometry and
J Volz1, F U Bosch, M Wunderlin
1Faculty of Chemistry, University of Konstanz, Germany.
Journal of Chromatography. A
|April 30, 1998
Abstract:
Metal ion-binding of synthetic peptides containing HxH and CxxC motifs was investigated by electrospray ionization mass spectrometry (ESI-MS) and metal chelate affinity chromatography. A high affinity of Ni2+ and Cu2+ to HxH containing sequences was found. Based on their natural metal ion-binding potential it was possible to include metal affinity chromatography in the purification process of two proteins without using an additional His-tag sequence: ATPase-439, a P type ATPase from Helicobacter pylori and the amyloid precursor protein (APP).