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[Biological response modifier activity of Lactococcus lactis 332]
1Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Summary
Heat-killed Lactococcus lactis 332 acts as a biological response modifier (BRM). This preparation stimulates immune cells, including macrophages, to produce tumor necrosis factor (TNF) and exhibits antitumor effects.
Area of Science:
- Immunology
- Microbiology
- Biotechnology
Background:
- Biological response modifiers (BRMs) are crucial in immunotherapy.
- Lactococcus lactis is known for producing lactate and has potential immunomodulatory properties.
Purpose of the Study:
- To investigate the biological response modifier (BRM) activity of heat-killed Lactococcus lactis 332.
- To evaluate its effects on immune cell activation and antitumor properties.
Main Methods:
- Administration of Lactococcus lactis 332 preparation to C3H/He and ICR mice via intraperitoneal injection.
- Assay of neutrophil and macrophage accumulation in the peritoneal cavity.
- Measurement of tumor necrosis factor (TNF) production by macrophages in vitro and in vivo.
- Assessment of antitumor activity via intratumoral injection in MM46 tumor-bearing mice.
Main Results:
- Lactococcus lactis 332 induced neutrophil and macrophage accumulation in the peritoneal cavity.
- The preparation demonstrated both priming and triggering activities for TNF production by macrophages.
- Significant TNF levels (approx. 1000 IU/ml) were detected in mouse sera 2 hours post-injection.
- Intratumoral administration led to regression of MM46 tumor cells.
Conclusions:
- Heat-killed Lactococcus lactis 332 functions as a potent biological response modifier (BRM).
- It activates phagocytes, stimulates TNF production, and exhibits significant antitumor activity.
- These properties suggest potential therapeutic applications, similar to existing BRMs like OK432.