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Near-field fluorescence microscopy of cells
1Molecular Design Institute, Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Ultramicroscopy
|May 5, 1998
Abstract:
We demonstrate the surface sensitivity of near-field scanning optical microscopy by fluorescence imaging of membrane and bulk proteins in cells. We discuss instrument design considerations for successful cell-biology work with NSOM and show that the technique is most suited for studying membrane proteins.