Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Forward and reverse genetic approaches to synaptogenesis

K Broadie1

  • 1Department of Biology, University of Utah, Salt Lake City 84112-0840, USA. broadie@biology.utah.edu

Current Opinion in Neurobiology
|May 6, 1998
PubMed
Summary

Genetic research on the neuromuscular synapse reveals new proteins crucial for synaptogenesis. Combining forward and reverse genetics in mice and Drosophila advances our understanding of synaptic maturation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stoned B mediates sorting of integral synaptic vesicle proteins.

Neuroscience·2008
Same author

Temperature-sensitive paralytic mutants: insights into the synaptic vesicle cycle.

Biochemical Society transactions·2006
Same author

Drosophila fragile X-related gene regulates the MAP1B homolog Futsch to control synaptic structure and function.

Cell·2001
Same author

Targeted mutations in the syntaxin H3 domain specifically disrupt SNARE complex function in synaptic transmission.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2001
Same author

Drosophila CAPS is an essential gene that regulates dense-core vesicle release and synaptic vesicle fusion.

Neuron·2001
Same author

Drosophila alpha- and beta-spectrin mutations disrupt presynaptic neurotransmitter release.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2001

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Synaptogenesis, the formation of synapses, is a complex process critical for nervous system function.
  • Understanding the molecular mechanisms underlying synaptic maturation is essential for addressing neurological disorders.

Purpose of the Study:

  • To investigate the mechanisms of synaptogenesis using genetic approaches.
  • To identify and analyze novel proteins involved in synaptic maturation.
  • To functionally characterize synaptic proteins in vivo.

Main Methods:

  • Utilizing classical (forward) and reverse genetic techniques.
  • Employing mouse reverse genetics.
  • Leveraging Drosophila forward genetics.

Main Results:

  • Isolation and analysis of novel proteins implicated in synaptic maturation.
  • Functional characterization of previously identified synaptic proteins in vivo.

Conclusions:

  • Genetic strategies, particularly mouse reverse genetics and Drosophila forward genetics, are powerful tools for dissecting synaptogenesis.
  • These approaches facilitate the discovery and functional validation of key players in synaptic development and function.

Related Experiment Videos