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Supernatant from stored red cells activates neutrophils
I Chin-Yee1, M Keeney, L Krueger
1Department of Hematology, London Health Sciences Centre, University of Western Ontario, Canada. ian.chinyee@LHSC.ON.CA
Transfusion Medicine (Oxford, England)
|May 7, 1998
Summary
Stored red blood cell (RBC) supernatant activates neutrophils, increasing adhesion molecule expression and oxidative burst. Leukodepletion mitigates some effects, suggesting other factors influence neutrophil priming.
Area of Science:
- Transfusion Medicine
- Immunology
- Hematology
Background:
- Bioreactive substances accumulate in stored red blood cells (RBCs).
- The clinical significance of these substances on neutrophil activity remains unclear.
Purpose of the Study:
- To investigate the in vitro effect of stored RBC supernatant on neutrophil activity.
- To assess the impact of leukodepletion on these effects.
Main Methods:
- Packed RBCs (PRBCs) were collected and divided into leukodepleted and non-leukodepleted aliquots.
- Supernatant was collected on various storage days (1-35).
- Neutrophil CD11b, CD16 expression, and oxidative burst were measured via flow cytometry; IL-8 and TNF-alpha levels were quantified.
Main Results:
- Supernatant from PRBCs stored >15 days activated neutrophils, increasing CD11b/CD16 expression and oxidative burst.
- The oldest units (35 days) showed the most significant neutrophil activation (P < 0.008).
- Leukodepletion reduced CD11b/CD16 expression changes (P < 0.02) but not oxidative burst priming (P > 0.1).
Conclusions:
- Stored PRBC supernatant contains factors that enhance neutrophil adhesion molecule expression and prime oxidative burst.
- Low levels of IL-8 and TNF-alpha suggest other substances are primarily responsible for neutrophil priming and activation.