Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Three-dimensional extracellular matrix engineering in the nervous system

M Borkenhagen1, J F Clémence, H Sigrist

  • 1Division of Surgical Research, Centre Hospitalier Universitaire Vaudois, Lausanne University Medical School, Switzerland.

Journal of Biomedical Materials Research
|May 7, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Oligodendrocyte gene expression is reduced by and influences effects of chronic social stress in mice.

Genes, brain, and behavior·2018
Same author

Altered emotionality and neuronal excitability in mice lacking KCTD12, an auxiliary subunit of GABAB receptors associated with mood disorders.

Translational psychiatry·2015
Same author

Rab1A over-expression prevents Golgi apparatus fragmentation and partially corrects motor deficits in an alpha-synuclein based rat model of Parkinson's disease.

Journal of Parkinson's disease·2013
Same author

An in vivo ultrahigh field 14.1 T (1) H-MRS study on 6-OHDA and α-synuclein-based rat models of Parkinson's disease: GABA as an early disease marker.

NMR in biomedicine·2012
Same author

Imaging brain amyloid deposition using grating-based differential phase contrast tomography.

NeuroImage·2012
Same author

Sustained expression of PGC-1α in the rat nigrostriatal system selectively impairs dopaminergic function.

Human molecular genetics·2012
Same journal

Optimizing the tensile properties of polyvinyl alcohol hydrogel for the construction of a bioprosthetic heart valve stent.

Journal of biomedical materials research·2002
Same journal

Wear of conventional and cross-linked ultra-high-molecular-weight polyethylene acetabular cups against polished and roughened CoCr femoral heads in a biaxial hip simulator.

Journal of biomedical materials research·2002
Same journal

Immobilization of natural macromolecules on poly-L-lactic acid membrane surface in order to improve its cytocompatibility.

Journal of biomedical materials research·2002
Same journal

Light scattering and in vitro biocompatibility studies of poly (vinyl pyrrolidone) derivatives with amino-acid-dependent groups.

Journal of biomedical materials research·2002
Same journal

Immobilization of an oxalate-degrading enzyme on silicone elastomer.

Journal of biomedical materials research·2002
Same journal

An evaluation of the cytotoxic effects of orthodontic bonding adhesives upon a primary human oral gingival fibroblast culture and a permanent, human oral cancer-cell line.

Journal of biomedical materials research·2002
See all related articles

This study developed a 3D hydrogel mimicking the extracellular matrix (ECM) to support nerve regeneration. The CDPGYIGSR peptide within the hydrogel successfully promoted neurite outgrowth in vitro and in vivo.

Area of Science:

  • Biomaterials Science
  • Neuroscience
  • Tissue Engineering

Background:

  • Neurite outgrowth during development and regeneration is guided by cellular and extracellular matrix (ECM) molecular cues.
  • Mimicking cell-matrix interactions is crucial for developing effective nerve regeneration strategies.
  • Three-dimensional (3D) hydrogels offer a promising platform for creating bioartificial ECM environments.

Purpose of the Study:

  • To design and evaluate a 3D hydrogel-based ECM equivalent for nerve regeneration.
  • To investigate the efficacy of a covalently immobilized laminin oligopeptide sequence (CDPGYIGSR) in supporting neurite outgrowth.
  • To assess the in vitro and in vivo performance of the developed hydrogel for enhancing nerve regeneration.

Main Methods:

  • Covalent immobilization of the laminin oligopeptide sequence CDPGYIGSR onto an agarose gel to create a 3D substrate.

Related Experiment Videos

  • In vitro assessment of neurite outgrowth from dorsal root ganglia (DRG) cultured on the derivatized gel.
  • Specificity testing using solubilized CDPGYIGSR peptide and control peptides (CDPGYIGSK, CDPGRGSYI).
  • In vivo evaluation in a transected rat dorsal root model, comparing CDPGYIGSR gel with controls (underivatized gel, CDPGRGSYI gel, saline channels).

    • Main Results:

    • The CDPGYIGSR-derivatized agarose gel successfully supported neurite outgrowth from DRG in vitro.
    • Neurite outgrowth was specifically promoted by CDPGYIGSR, as evidenced by inhibition in the presence of excess soluble peptide.
    • Gels with control peptides (CDPGYIGSK, CDPGRGSYI) showed a smaller increase in neurite outgrowth.
    • In vivo, the CDPGYIGSR gel significantly enhanced nerve regeneration in a rat model compared to all control groups.

    Conclusions:

    • A 3D hydrogel-based ECM equivalent functionalized with the CDPGYIGSR peptide effectively supports neurite outgrowth.
    • This bioartificial substrate demonstrates significant potential for enhancing nerve regeneration both in vitro and in vivo.
    • The findings suggest the feasibility of using such engineered hydrogels for therapeutic nerve repair strategies.