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Filamentous phage fs1 of Vibrio cholerae O139
1Department of Bacteriology, University of the Ryukyus, Faculty of Medicine, Okinawa, Japan. noboru@med.u-ryukyu.ac.jp
Microbiology and Immunology
|May 7, 1998
Abstract:
Filamentous phage, fs1, was obtained from Vibrio cholerae O139. The lysogenized strains produced a large amount of fs1 phage in the culture supernatant. This phage was previously reported as novel fimbriae of that organism. The genome of the phage was a 6.5 kb single-stranded DNA. The capsid of fsl consists of a small molecule peptide (about 2.5 kDa).
Insights
Filamentous phage fs1 was isolated from Vibrio cholerae O139. This novel phage, previously identified as fimbriae, has a single-stranded DNA genome and a small peptide capsid.
Area of Science:
- Microbiology
- Virology
- Molecular Biology
Background:
- Filamentous phage fs1 was isolated from Vibrio cholerae O139.
- This phage was previously characterized as novel fimbriae.
Purpose of the Study:
- To characterize the filamentous phage fs1 obtained from Vibrio cholerae O139.
- To analyze the genomic and structural properties of fs1 phage.
Main Methods:
- Isolation of filamentous phage fs1 from Vibrio cholerae O139.
- Analysis of phage genome size and structure.
- Characterization of phage capsid protein.
Main Results:
- Lysogenized Vibrio cholerae O139 strains produced large quantities of fs1 phage.
- The fs1 phage genome consists of 6.5 kb single-stranded DNA.
- The fs1 phage capsid is composed of a small peptide (approximately 2.5 kDa).
Conclusions:
- Filamentous phage fs1 is a novel phage associated with Vibrio cholerae O139.
- fs1 phage possesses a single-stranded DNA genome and a simple protein capsid.