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Sequence of a cDNA encoding mouse ribosomal protein S14
1Department of Genetic Engineering, College of Agriculture, Chonnam National University, Kwangju, Korea.
Bioscience, Biotechnology, and Biochemistry
|May 8, 1998
Summary
Researchers identified a mouse mammary gland clone encoding ribosomal protein S14 (rps14). This ribosomal protein showed significant sequence conservation across multiple species, highlighting its importance in cellular function.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Ribosomal proteins are essential components of ribosomes, crucial for protein synthesis.
- Gene expression and protein function can be studied using cDNA libraries and differential screening.
- Mammary gland development involves complex gene regulation.
Purpose of the Study:
- To identify novel genes involved in mammary gland involution.
- To characterize a specific clone identified through differential screening.
- To analyze the evolutionary conservation of the identified gene product.
Main Methods:
- Differential screening of a mouse mammary gland cDNA library.
- Full-length cDNA sequencing.
- Amino acid sequence comparison with homologous proteins from other species.
Main Results:
- An involution-induced clone was isolated and identified.
- The clone represents the full-length cDNA encoding the 40S ribosomal protein S14 (rps14).
- The deduced amino acid sequence of mouse rps14 is highly conserved in humans, hamsters, and rats.
Conclusions:
- Ribosomal protein S14 (rps14) is regulated during mouse mammary gland involution.
- The high conservation of rps14 suggests a critical and conserved role in cellular function across mammals.
- This finding provides a basis for further functional studies of rps14 in mammary gland biology and beyond.