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Differentiation of Cryptosporidium parvum isolates by a simplified randomly amplified polymorphic DNA technique
1Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis 95616, USA.
Applied and Environmental Microbiology
|May 9, 1998
Abstract:
Genomic DNA was isolated from Cryptosporidium parvum oocysts by a specific immunomagnetic separation-in vitro excystation procedure and subjected to randomly amplified polymorphic DNA analysis using sequence-independent primers. An estuary C. parvum isolate was easily differentiated from several bovine isolates, while five bovine isolates of the same origin were indistinguishable from each other.